Coding

Part:BBa_K3425034:Design

Designed by: Tereza Hubackova   Group: iGEM20_UofUppsala   (2020-10-18)


T7 RNA polymerase for Type IIS standard (RFC1000)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is designed for the Type IIS iGEM standard assembly and therefore the coding sequence lacks the start codon. In Type IIS standard assembly, the ATG start codon is included in the coding-sequence prefix. You can find useful design templates and more information about how to use this standard in the Type IIS guidebook of Uppsala 2020 team.

Source

Isolated from the genomic DNA of the E. coli BL21(DE3)pLysS strain using custom primers.

References